Dengue Serotyping Virus

The Dengue Serotyping system is a qualitative test that allows the RNA amplification in Real-Time PCR of anchored capsid protein region of Dengue Virus (DENV), discriminating the 4 serotypes: DENV-1, DENV-2, DENV-3 and DENV-4.

The Procedure allows the detection of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

Target:

RNA corrisponding to the anchored capsid protein region of Dengue Virus (DENV)
Internal Control (IC)

Diagnostic samples:

Serum
Plasma

Real-Time PCR instruments:

7500 Fast from Lifetechnologies
Rotor-Gene Q MDx from QIAGEN

RNA Extraction:

Manual Extraction with:

QIAmp Viral RNA Mini Kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)