Malaria Screening

The system Malaria screening is a qualitative test that allows the DNA amplification and detection, by means of Real-Time PCR, of P.falciparum, P.malariae, P.vivax, P.ovale and P. knowlesi genome extracted from biological samples, without species discrimination.

The Procedure allows the detection of the DNA target by means a genomic amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyser (thermal cycler integrated with a system for fluorescence detection and a dedicated software)

Diagnostic samples:

Whole Blood EDTA

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (QIAGEN)
Versant kPCR AD (Siemens)
CFX96 Real-Time PCR Detection System (Bio-Rad)

RNA Extraction:

Manual Extraction with:

QIAmp DNA mini kit (QIAGEN)
VERSANT® Sample Preparation 1.2 Reagents (SIEMENS Healthineers)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
MagPurix 12A (ZINEXTS LIFE SCIENCE CORP)