Beta Fibrinogen

DuplicαRealTime Beta-Fibrinogen 455G>A Genotyping Kit is an in vitro diagnostic test for the detection, by Real-Time PCR, of the allelic variant 455G>A in the promoter region of the Human Beta-Fibrinogen gene, in genomic DNA

Overview

Increased plasma fibrinogen levels have been identified as a risk indicator for myocardial infarction, stroke, and thrombosis. Both environmental and genetic factors make an important contribution to plasma fibrinogen levels in humans. Fibrinogen (Factor I) is a glycoprotein, synthesized in the liver, that participates in the coagulation cascade.

Fibrinogen converts to fibrin in the presence of thrombin. Elevated fibrinogen levels have been associated with increased risk for atherosclerosis and deep venous thrombosis as well as cardiovascular diseases. Fibrinogen is composed of three pairs of polypeptide chains (named α, β and γ) which are linked by disulfide bonds. These three chains are encoded by three different genes (α, β and γ, respectively), that are located on chromosome 4. Among genetic factors, a common G → A polymorphism at position - 455 in the promoter region of the Beta -Fibrinogen gene has been associated with elevated fibrinogen levels (for the AA homozygous carriers).

Targets:

Allelic variant 455G>A in the promoter region of the Human Beta-Fibrinogen gene, in genomic DNA
Internal Control (IC)

Diagnostic samples:

Peripheral whole blood samples collected in EDTA

DNA Extraction:

Compatible with the most common DNA Extraction and Purification Systems

Real-Time PCR instruments:

Compatible with the most common Real-Time PCR Systems