48/96 Tests - ON0123
Gluten DQuick DResolution v4
Gluten DQuick DResolution v4.0 – Advanced HLA Typing Kit for Celiac Disease
The Gluten DQuick DResolution v4 system is an in vitro molecular diagnostic test for the detection of of HLA haplotypes DQ2 (DQ2.2 and DQ2.5), DQ8, DQ7.5, DQ2.3, DR3, DR4, DR7, DR11 and DR12, by Real-Time PCR.
Celiac disease is a chronic autoimmune enteropathy triggered by gluten ingestion in genetically predisposed individuals, leading to small intestinal damage and malabsorption.
Early identification of genetic predisposition is a key step in optimizing the diagnostic workflow and avoiding unnecessary invasive testing.
HLA-DQ2 and DQ8 class II heterodimers account for approximately 40% of celiac disease heritability, while overall heritability—including non-HLA genes—is estimated at around 75%. The DQ2.5 haplotype (DQA1*05 + DQB1*02) confers the highest genetic risk, with DQ2.2 (DQA1*02 + DQB1*02) and DQ8 (DQA1*03 + DQB1*03:02) also significantly associated. These haplotypes are present in over 95% of celiac patients, making their absence a powerful negative predictor of disease (<5% chance).
Gluten DQuick DResolution v4.0 is Clonit’s most advanced HLA typing kit for the genetic assessment of celiac disease predisposition.
The test amplifies and detects the following HLA class II alleles:
- DQA1*02, DQA1*03, DQA1*05
- DQB1*02, DQB1*03:01, DQB1*03:02
- DRB1*03, DRB1*04, DRB1*05, DRB1*07
This comprehensive panel allows the identification of key celiac disease risk haplotypes:
- DQ2 (DQ2.2, DQ2.3, DQ2.5)
- DQ7.5
- DQ8
The detection of DR3, DR4, DR5, and DR7 haplotypes — in linkage disequilibrium with DQ alleles — improves result validation and ensures reliable typing.
Why choose Gluten DQuick DResolution v4.0:
- High-resolution detection of DQ2 and DQ8 homozygosity
- Fully automated haplotype assignment with dedicated, user-friendly software
- Developed in accordance with the AIC (Italian Celiac Association) clinical recommendations for HLA genetic testing (Congia M., 2022)
- CE-IVDR marked, compliant with the latest European regulations for in vitro diagnostic medical devices
- Ideal for clinical laboratories performing celiac disease genetic testing and risk stratification
Clinical relevance:
Gluten DQuick DResolution v4.0 enables accurate risk stratification, guiding follow-up of at-risk individuals and helping avoid unnecessary invasive procedures. Its comprehensive allele coverage and automated interpretation software support gastroenterologists and laboratory professionals in the diagnosis and management of patients with suspected celiac disease.
Biological samples:
- Blood EDTA
- Buccal swab
Real-Time PCR Instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
- CFX96 Real-Time PCR Detection System (Bio-Rad)