Rhyma Test IVF AZF

RHYMA TEST IVF AZF STRIP TEST is an in vitro diagnostic assay for the multiplex detection of the Sequence Tagged Sites (STSs) microdeletions into the AZF gene regions: AZFa region (sY84, sY86); AZFb region (sY127, sY134) and AZFc region (sY254 and sY255) in human DNA, by Reverse Hybridization Technology.

The assay detects also the ZFX/ZFY gene (as internal control) and SRY gene (as additional control).

Overview

Infertility is a major health problem affecting 10-15% of couples seeking to have children and a male factor can be identified in about half of these cases. A significant proportion of infertile males are affected either by oligozoospermia or azoospermia.

The recent growth of assisted reproduction techniques has led to the identification of Y chromosome microdeletions into AZF regions correlated to non-obstructive azoospermia, Men with deletions in AZFa and AZFb regions show severe defects in spermatogenesis, whereas deletions of AZFc region can be compatible with residual spermatogenesis.

This Clonit test is based on Gel Electrophoresis and Reverse Hybridization protocol and it has been developed in agreement with guidelines of European Molecular Genetics Quality Network (EMQN) 2004.

Targets:

AZFa region (sY84, sY86);
AZFb region (sY127, sY134)
AZFc region (sY254 and sY255)
Internal Control (IC)

Diagnostic samples:

Blood samples

DNA Extraction:

Compatible with the most common DNA Extraction and Purification Systems

PCR instruments:

Compatible with the most common PCR Systems

RHYMA TEST IVF AZF STRIP TEST is also available for automatic protocols (Ref. EES003020D)