ZEBOV - Ebolavirus Zaire Strain
Code
RT-35
CE Mark
RUO
Tests

48 tests

ZEBOV - Ebolavirus Zaire Strain

The quanty ZEBOV system is a quantitative test that allows the RNA reverse transcription, amplification and quantification, by Real-Time PCR, of NP (Nucleoprotein) gene of Ebola genome.

The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

 

Target:

  • NP gene of Ebola genome
  • Internal Control (IC)

Standard for quantification:

  • synthetic RNA corresponding to NP gene 1.000.000 cps/µL
  • synthetic RNA corresponding to NP gene 100.000 cps/µL
  • synthetic RNA corresponding to NP gene 1.0000 cps/µL
  • synthetic RNA corresponding to NP gene 1.000 cps/µL

Diagnostic samples:

  • Serum
  • Plasma

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

  • StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)

RNA Extraction:

Manual Extraction with:

  • QIAmp Viral RNA Mini Kit (QIAGEN)