quanty ZIKA

The quanty Zika system is a quantitative test that allows the RNA amplification and quantification, by means of Real-Time PCR, of NS5 region of Zika Virus RNA.

The Procedure allows the detection and quantification of the RNA target by means of a retro-amplification reaction. The analysis of the results is made using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

Target:

NS5 region of Zika virus RNA
Internal Control (IC)

Standard for quantification:

synthetic RNA corresponding to NS5 region 100.000 cps/ml
synthetic RNA corresponding to NS5 region 10.000 cps/µL
synthetic RNA corresponding to NS5 region 1.000 cps/µL
synthetic RNA corresponding to NS5 region 100 cps/µL

Diagnostic samples:

Serum
Saliva
Urine

Real-Time PCR instrument:

Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

RNA Extraction:

Manual Extraction with:

QIAmp Viral RNA Mini Kit (QIAGEN)