Dengue Serotyping Virus
The Dengue Serotyping system is a qualitative test that allows the RNA amplification in Real-Time PCR of anchored capsid protein region of Dengue Virus (DENV), discriminating the 4 serotypes: DENV-1, DENV-2, DENV-3 and DENV-4.
The Procedure allows the detection of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).
- RNA corrisponding to the anchored capsid protein region of Dengue Virus (DENV)
- Internal Control (IC)
Real-Time PCR instruments:
- 7500 Fast from Lifetechnologies
- Rotor-Gene Q MDx from QIAGEN
Manual Extraction with:
- QIAmp Viral RNA Mini Kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)
- QIAsymphony SP (QIAGEN)