Code
RT-05
CE MarkRT-05
Tests

48 test

quanty Chikungunya

The quanty Chikungunya system is a quantitative test that allows the RNA amplification in Real-Time PCR, of nsP2 region of Chikungunya (CHIK).

The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

 

Target:

  • nsP2 region of Chikungunya
  • Internal Control (IC)

Standard for quantification:

  • synthetic RNA corresponding to nsP2 region 100.000 cps/µL
  • synthetic RNA corresponding to nsP2 region 10.000 cps/µL
  • synthetic RNA corresponding to nsP2 region 1.000 cps/µL
  • synthetic RNA corresponding to nsP2 region 100 cps/µL

Diagnostic samples:

  • Serum
  • Plasma

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

RNA Extraction:

Manual Extraction with:

  • QIAmp Viral RNA Mini Kit (QIAGEN)

Automatic Extraction with:

  • EZ1 Advanced XL (QIAGEN)
  •  QIAsymphony SP (QIAGEN)