quanty Usutu
Code
RT-20
CE MarkRT-20
Tests

48 Tests

quanty Usutu

The quanty Usutu system is a quantitative test that allows the RNA amplification, by means of Real-Time PCR, of 3’UTR region of Usutu Virus (USUV).

The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

 

Target:

  •  3’UTR region of Usutu Virus (USUV)
  • Internal Control (IC)

Standard for quantification:

  • synthetic RNA corresponding to 3’UTR region of USUV 100.000 cps/µL
  • synthetic RNA corresponding to 3’UTR region of USUV 10.000 cps/µL
  • synthetic RNA corresponding to 3’UTR region of USUV 1.000 cps/µL
  • synthetic RNA corresponding to 3’UTR region of USUV 100 cps/µL

Diagnostic samples:

  • Serum
  • Urine

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

  • LightCycler® 480 Real-Time PCR System (Roche)

RNA Extraction:

Manual Extraction with:

  • QIAmp Viral RNA Mini Kit (QIAGEN)

Automatic Extraction with:

  • EZ1 Advanced XL (QIAGEN)
  •  QIAsymphony SP (QIAGEN)