Code

RT-20

CE Mark

Tests

48 Tests

quanty Usutu

The quanty Usutu system is a quantitative test that allows the RNA amplification, by means of Real-Time PCR, of 3’UTR region of Usutu Virus (USUV).

The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

Target:

•  3’UTR region of Usutu Virus (USUV)
• Internal Control (IC)

Standard for quantification:

• synthetic RNA corresponding to 3’UTR region of USUV 100.000 cps/µL
• synthetic RNA corresponding to 3’UTR region of USUV 10.000 cps/µL
• synthetic RNA corresponding to 3’UTR region of USUV 1.000 cps/µL
• synthetic RNA corresponding to 3’UTR region of USUV 100 cps/µL

Diagnostic samples:

• Serum
• Urine

Real-Time PCR instruments:

• Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
• Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

• LightCycler® 480 Real-Time PCR System (Roche)

RNA Extraction:

Manual Extraction with:

• QIAmp Viral RNA Mini Kit (QIAGEN)

Automatic Extraction with:

• EZ1 Advanced XL (QIAGEN)
•  QIAsymphony SP (QIAGEN)