ZEBOV - Ebolavirus Zaire Strain

The quanty ZEBOV system is a quantitative test that allows the RNA reverse transcription, amplification and quantification, by Real-Time PCR, of NP (Nucleoprotein) gene of Ebola genome.

The Procedure allows the detection and quantification of the RNA target by means a One step retro-amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyzer (thermal cycler integrated with a system for fluorescence detection and a dedicated software).

Target:

• NP gene of Ebola genome
• Internal Control (IC)

Standard for quantification:

• synthetic RNA corresponding to NP gene 1.000.000 cps/µL
• synthetic RNA corresponding to NP gene 100.000 cps/µL
• synthetic RNA corresponding to NP gene 1.0000 cps/µL
• synthetic RNA corresponding to NP gene 1.000 cps/µL

Diagnostic samples:

• Serum
• Plasma

Real-Time PCR instruments:

• Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
• Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

• StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)

RNA Extraction:

Manual Extraction with:

• QIAmp Viral RNA Mini Kit (QIAGEN)