Leishmania spp
Code
RT-63
CE MarkRT-63
Tests

48 Test

Leishmania spp

The system Leishmaniae spp.is a qualitative test that allows the DNA amplification in Real-Time PCR, of Leishmaniae genome extracted from biological samples.

The procedure allows the detection of the DNA target (18s Ribosomal RNA gene) through a genomic amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyser (thermal cycler integrated with a system for fluorescence detection and  a dedicated software)

Targets:

  • 18s Ribosomal RNA gene
  • Internal Control (IC)

Diagnostic samples:

  • Whole Blood EDTA

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (QIAGEN)
  • Versant kPCR AD (Siemens) 
  • CFX96 Real-Time PCR Detection System (Bio-Rad)
  • LightCycler® 480 Real-Time PCR System (Roche)

RNA Extraction:

Manual Extraction with:

  • QIAmp DNA mini kit (QIAGEN)
  • VERSANT® Sample Preparation 1.2 Reagents (SIEMENS Healthineers)

Automatic Extraction with:

  • EZ1 Advanced XL (QIAGEN)