Code
RT-66
CE MarkRT-66
Tests

48 Test

Malaria Screening

The system Malaria screening is a qualitative test that allows the DNA amplification and detection, by means of Real-Time PCR, of P.falciparum, P.malariae, P.vivax, P.ovale and P. knowlesi genome extracted from biological samples, without species discrimination.

The Procedure allows the detection of the DNA target by means a genomic amplification reaction. The analysis of the results is obtained using a Real-Time PCR analyser (thermal cycler integrated with a system for fluorescence detection and  a dedicated software)

 

Diagnostic samples:

  • Whole Blood EDTA

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (QIAGEN)
  • Versant kPCR AD (Siemens) 
  • CFX96 Real-Time PCR Detection System (Bio-Rad)
  • mic qPCR cycler (Bio Molecular System)

RNA Extraction:

Manual Extraction with:

  • QIAmp DNA mini kit (QIAGEN)
  • VERSANT® Sample Preparation 1.2 Reagents (SIEMENS Healthineers)

Automatic Extraction with:

  • EZ1 Advanced XL (QIAGEN)
  • MagPurix 12A (ZINEXTS LIFE SCIENCE CORP)