quanty HEV

The quanty HEV system is an in vitro diagnostic test for identification and quantification of Hepatitis E genome, by one-step RT-PCR Real-Time.

Hepatitis E is a liver infection caused by the hepatitis E virus (HEV). HEV is found in the stool of an infected person. It is spread when someone unknowingly ingests the virus – even in microscopic amounts. In developing countries, people most often get hepatitis E from drinking water contaminated. In the United States and other developed countries where hepatitis E is not common, people have gotten sick with hepatitis E after eating raw or undercooked meats. Symptoms of hepatitis E can include fatigue, poor appetite, stomach pain, nausea, and jaundice. However, many people with hepatitis E, especially young children, have no symptoms. Except for the rare occurrence of chronic hepatitis E in people with compromised immune systems, most people recover fully from the disease without any complications. No vaccine for hepatitis E is currently available. The most sensitive method for confirming a diagnosis of HEV is the use of Real-Time PCR.

Target:

ORF3 region of HEV genome
Internal Control (IC)

Standard for quantification:

Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

Plasma
Serum

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
QuantStudio™ 5 Real-Time PCR Instrument (ThermoFisher SCIENTIFIC)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
LightCycler® 480 Real-Time PCR System (Roche)
Versant kPCR AD (Siemens)

RNA Extraction:

Manual Extraction with:

QIAmp Viral RNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)