quanty HDV

The quanty HDV system is a molecular assay for identification and quantification of Hepatitis D genome, by one-step RT-PCR Real-Time. 

Hepatitis D, also known as “delta hepatitis,” is a liver infection caused by the hepatitis D virus (HDV). Hepatitis D only occurs in people who are also infected with the hepatitis B virus. Hepatitis D is spread when blood or other body fluids from a person infected with the virus enters the body of someone who is not infected. Hepatitis D can be an acute, short-term infection or become a long-term, chronic infection. Hepatitis D can cause severe symptoms and serious illness that can lead to life-long liver damage and even death. The most sensitive method for confirming a diagnosis of HDV is the use of Real-Time PCR.

Target:

• LHD gene of HDV genome
• Internal Control (IC)

Standard for quantification:

• Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

• Plasma
• Serum

Real-Time PCR instruments:

• Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
• Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)

RNA Extraction:

Manual Extraction with:

• QIAmp Viral RNA mini kit  (QIAGEN)

Automatic Extraction with:

• EZ1 Advanced XL (QIAGEN)