The quanty HAV system is an in vitro diagnostic test for identification and quantification of Hepatitis A genome, by one-step RT-PCR Real-Time.
Hepatitis A is a vaccine-preventable liver infection caused by the hepatitis A virus (HAV). HAV is found in the stool and blood of people who are infected. Hepatitis A is very contagious. It is spread when someone unknowingly ingests the virus — even in microscopic amounts — through close personal contact with an infected person or through eating contaminated food or drink. Symptoms of hepatitis A can last up to 2 months and include fatigue, nausea, stomach pain, and jaundice. Most people with hepatitis A do not have long-lasting illness. The best way to prevent hepatitis A is to get vaccinated. The most sensitive method for confirming a diagnosis of HAV is the use of Real-Time PCR.
- 5'-UTR of HAV genome
- Internal Control (IC)
Standard for quantification:
- Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)
Real-Time PCR instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
Manual Extraction with:
- QIAmp Viral RNA mini kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)