The quanty HDV system is a molecular assay for identification and quantification of Hepatitis D genome, by one-step RT-PCR Real-Time.
Hepatitis D, also known as “delta hepatitis,” is a liver infection caused by the hepatitis D virus (HDV). Hepatitis D only occurs in people who are also infected with the hepatitis B virus. Hepatitis D is spread when blood or other body fluids from a person infected with the virus enters the body of someone who is not infected. Hepatitis D can be an acute, short-term infection or become a long-term, chronic infection. Hepatitis D can cause severe symptoms and serious illness that can lead to life-long liver damage and even death. The most sensitive method for confirming a diagnosis of HDV is the use of Real-Time PCR.
- LHD gene of HDV genome
- Internal Control (IC)
Standard for quantification:
- Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)
Real-Time PCR instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
Manual Extraction with:
- QIAmp Viral RNA mini kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)