ISAba1-blaOXA51-like junction and blaOXA-58, blaOXA-23, blaOXA-24, blaOXA-143 genes
A.baumanii Genotyping
Duplicα RealTime MDR A. baumannii OXA Genotyping Kit is an in vitro diagnostic test for the detection of of the most common OXA genes conferring antibiotic resistance to Acinetobacter baumannii: blaOXA-58, blaOXA-23, blaOXA-24, blaOXA-143 genes and the ISAba1-blaOXA-51-like junctions, by Real-Time PCR.
Overview
Multidrug-resistant Acinetobacter baumannii is recognized to be one of the most hardly-treatable and controllable antimicrobial-resistant Gram-negative bacilli. Increasing antimicrobial resistance among Acinetobacter isolates has been documented, although several definitions of multidrug resistance are reported in the literature. A. baumannii survives for prolonged periods under a wide range of environmental conditions. The organism causes outbreaks of infection and health care–associated infections, including bacteremia, pneumonia, meningitis, urinary tract infection and wound infection. The management of this pathogen has become a significant challenge due to the increased emergence of carbapenem-resistant strains. It has been shown that most of the carbapenem resistance in A. baumannii is due to the production of carbapenemases, especially those belonging to Carbapenem-Hydrolyzing class D beta-Lactamases (CHDLs), which are encoded by the blaOXA-51-like, blaOXA-23-like, blaOXA-24-like, and blaOXA-58-like genes and the emerging blaOXA-143-like gene. Among these CHDL genes, the blaOXA-51-like gene is intrinsic to and is located on the chromosome of A. baumannii, while the other blaOXA genes are normally located on extrachromosomal plasmids and can migrate from one strain to another, conferring resistance to the host strains. The CHDLs have a weak carbapenem-hydrolyzing activity. However, the overexpression of these CHDL genes, mostly driven by promoters provided by their upstream Insertion Sequences (ISs), is one of the means by which A. baumannii acquires a high level of carbapenem resistance. For example ISAba1, upstream of the blaOXA-51-like gene, is associated with the overexpression of the blaOXA-51-like gene and the carbapenem resistance in A. baumannii, while the other blaOXA genes are normally overexpressed since they are located on plasmids that are usually present in multiple copies. Since the therapeutic options for the infection of Acinetobacter are limited, the development or discovery of new therapies, as well as methods that allow the analysis and identification of this bacterium in dedicated care facilities, are crucial to limit and prevent its spread. This kit discriminates between blaOXA-58, blaOXA-23, blaOXA-24, blaOXA-143 genes and the ISAba1-blaOXA-51-like junction. Moreover, the kit allows to identify the A. baumannii strain respect the Acinetobacter strains due to the recognition of blaOXA-51 gene.
Targets:
- blaOXA-51, blaOXA-24 genes, the ISAba1-blaOXA-51- like junction
- blaOXA-58, blaOXA-23, blaOXA-143 gene
- Internal Control (IC)
Diagnostic samples:
- Clinical samples
DNA Extraction:
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Compatible with the most common DNA Extraction and Purification Systems
Real-Time PCR instruments:
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Compatible with the most common Real-Time PCR Systems