The quanty Rubella system is a molecular assay for identification and quantification of the E1 region of Rubella virus genome, by one-step RT- Real-Time PCR.
Rubella is a contagious viral infection best known by its distinctive red rash. It's also called German measles or three-day measles. This infection may cause mild or no symptoms in most people. However, it can cause serious problems for unborn babies whose mothers become infected during pregnancy. Rubella is rare in many countries because most children are vaccinated against the infection at an early age. In some parts of the world, the virus is still active. This is something to consider before going abroad, especially if you're pregnant.
In case a pregnant woman falls ill with rubella, the effect on her unborn child may be severe, and in some cases, fatal. Up to 90% of infants born to mothers who had rubella during the first 12 weeks of pregnancy develop congenital rubella syndrome. This syndrome can cause one or more problems, including: growth delays, cataracts, deafness, problems with the development of the heart (congenital heart defects), problems with the development of other organs, problems with mental development and learning.
- E1 region of Rubella virus genome
- Internal Control (IC)
Standard for quantification:
- Synthetic RNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)
- Different clinical speciemens
Real-Time PCR instruments:
- Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
- Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
Manual Extraction with:
- QIAmp Viral RNA mini kit (QIAGEN)
Automatic Extraction with:
- EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)