Mycobacterium Complex (IS6110 region)

The MTB complex system is a qualitative in vitro diagnostic test for identification of Mycobacterium tuberculosis complex genome, by Real-Time PCR.

Target:

IS6110 region of MTB complex genome
Internal Control (IC)

Diagnostic samples:

Sputum
BAL
Expectorate

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
StepOnePlus™ Real-Time PCR System (ThermoFisher SCIENTIFIC)
LightCycler® 480 Real-Time PCR System (Roche)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
CFX96 Real-Time PCR Detection System (Bio-Rad)
Versant kPCR AD (Siemens)

DNA Extraction:

Manual Extraction with:

QIAmp DNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)