CE MarkRT-12

48 Tests - ON0318

Available for QiaSymphony SP/AP - Ref. QS-12


quanty CMV

The quanty CMV virus system is an in vitro diagnostic test for identification and quantification of the IEA region of Cytomegalovirus genome, by Real-Time PCR. 

In healthy individuals Cytomegalovirus infection is usually asymptomatic while it represents one of the most important infectious complications of solid-organ transplantation and it is responsible for serious, life-threatening diseases in patients infected with HIV. Furthermore, CMV is the most common congenital viral infection: both asymptomatic and symptomatic newborns are at risk of developing long-term neurodevelopmental morbidity, particularly sensorineural hearing loss. CMV disease manifests in the vast majority of transplant recipients as a viral syndrome that includes fever, malaise, myalgia or headache. CMV infection most commonly involves the transplanted organ but spreading and involvement of other organs, such as the central nervous system, the eye, and the urogenital or gastrointestinal tract, is frequently observed. The monitoring of CMV infection following solid-organ transplantation is of prime importance. Cytomegalovirus detection and quantification by Real-Time PCR is essential to quickly set up an appropriate antiviral treatment and prevent serious complications.


  • IEA region of CMV genome
  • Internal Control (IC)

Standard for quantification:

  • Synthetic DNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

  • Blood EDTA
  • Liquor (CSF)
  • Urine

Real-Time PCR instruments:

  • Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
  • Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
  • LightCycler® 480 Real-Time PCR System (Roche)
  • Versant kPCR AD (Siemens) 
  • CFX96 Real-Time PCR Detection System (Bio-Rad)

DNA Extraction:

Manual Extraction with:

  • QIAmp DNA mini kit  (QIAGEN)

Automatic Extraction with:

  • EZ1 Advanced XL (QIAGEN)
  • QIAsymphony SP (QIAGEN)