quanty Parvo B19

The quanty Parvo B19 system is an vitro diagnostic test for identification and quantification of Parvovirus B19 genome, by Real-Time PCR.

Parvovirus infection is a common and highly contagious childhood illness. It's sometimes called slapped-cheek disease because of the distinctive face rash that develops. Parvovirus infection has also been known as fifth disease because, historically, it was fifth in a list of common childhood illnesses characterized by a rash. In most children, parvovirus infection is mild and needs little treatment. But in some adults, the infection can be serious. Parvovirus infection during pregnancy sometimes affects red blood cells in the fetus. Although uncommon, this may cause severe anemia that could lead to miscarriage or stillbirth. Fetal risk appears to be greatest during the first half of the pregnancy. The infection is also more serious for people with some kinds of anemia or who have a compromised immune system such as in case of HIV infection, cancer treatments and anti-rejection drugs used after organ transplants. Quantitative Parvo B19 Real-Time PCR test represents an indispensable diagnostic tool to recognize and monitoring this infection.

Target:

VP1 region of Parvovirus B19 genome
Internal Control (IC)

Standard for quantification:

Synthetic DNA corresponding to the target region (100.000 cps/µL - 100 cps/µL)

Diagnostic samples:

Whole Blood EDTA
Plasma EDTA

Real-Time PCR instruments:

Applied Biosystems 7500 Fast (ThermoFisher SCIENTIFIC)
LightCycler® 480 Real-Time PCR System (Roche)
Rotor-Gene Q MDx (RG-Q MDx - QIAGEN)
CFX96 Real-Time PCR Detection System (Bio-Rad)
Versant kPCR AD (Siemens)

DNA Extraction:

Manual Extraction with:

QIAmp DNA mini kit (QIAGEN)

Automatic Extraction with:

EZ1 Advanced XL (QIAGEN)
QIAsymphony SP (QIAGEN)